How does the overexpression of the swnR gene affect the expression of other SW biosynthesis-related genes in Alternaria oxytropis OW7.8?

Label:chem

Topic
The swnR gene is known to be involved in swainsonine (SW) biosynthesis in Alternaria oxytropis OW7.8. To understand the regulatory role of swnR, the expression levels of other SW biosynthesis-related genes were examined in the swnR overexpression strain (swnR-OE).

From: "The Effect of the swnR Gene on Swainsonine Biosynthesis in Alternaria oxytropis OW7.8, an Endophytic Fungus of Oxytropis glabra", Microorganisms 2025, 13(6), 1326;
Answer
RT-qPCR analysis showed that the overexpression of the swnR gene in A. oxytropis OW7.8 led to increased transcript levels of the sac, P5CR, swnK, and swnN genes, while the swnH1 gene expression was decreased, and the swnH2 gene expression remained unchanged. This indicates that the swnR gene positively regulates the expression of several key genes involved in SW biosynthesis, thereby influencing the overall SW production pathway.
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