What is the role of adenine in detecting bacterial stress responses using Surface-Enhanced Raman Spectroscopy (SERS)?
Label:chem
Topic
Adenine is a key metabolite indicative of stress conditions in bacteria. It can be detected in the bacterial cell and/or released into the environment as a signaling molecule. SERS is a technique that enhances the Raman signal by placing the sample in the proximity of plasmonic nanostructures, enabling more accurate identification of bacteria and their metabolites. This study investigates the metabolic responses of five selected bacterial strains to physiological stress by analyzing the release of adenine using SERS.
From: "Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions", Sensors 2025, 25(15), 4629;
Answer
Adenine serves as a stress marker in bacterial cells under stress conditions. SERS enables the detection of adenine released by bacteria under osmotic stress, which is not detectable by conventional Raman spectroscopy. This detection helps in understanding bacterial stress responses and metabolic activities.
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