How does osmotic stress induced by deionized (DI) water affect the detection of adenine in bacterial cells using SERS?

Label:chem

Topic
Osmotic stress is induced by exposing bacterial cells to deionized water, which causes changes in the bacterial metabolic profile. The study investigates the effect of this stress on the release of adenine and its subsequent detection using SERS.

From: "Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions", Sensors 2025, 25(15), 4629;
Answer
Osmotic stress induced by DI water leads to the release of adenine from bacterial cells, making it detectable by SERS. The study shows that under osmotic stress conditions, a distinct SERS peak at ~730 cm⁻¹ corresponding to adenine is observed, indicating active metabolite release under stress. This finding highlights the potential of SERS for monitoring bacterial stress responses in real-time.
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