How do ropivacaine and lidocaine affect mitochondrial function in osteosarcoma cells?

Mitochondrial dysfunction is a key hallmark of cancer, influencing both metabolic adaptation and apoptotic susceptibility. Local anesthetics have been shown to influence mitochondrial membrane potential and reactive oxygen species (ROS) generation in various cell types.

Both ropivacaine and lidocaine induce mitochondrial dysfunction in SaOS-2 cells by decreasing mitochondrial membrane potential, enhancing mitochondrial superoxide production, and causing pronounced mitochondrial fragmentation. These changes lead to increased oxidative stress and promote apoptosis through the intrinsic mitochondrial pathway.