What role does p-dimethylaminobenzaldehyde (DMAB) play in the spectrophotometric determination of cephalosporins?

Label:chem

Topic
The study explores a new spectrophotometric method for analyzing cephalosporins, which involves a diazotization reaction followed by coupling with p-dimethylaminobenzaldehyde (DMAB). DMAB is used as a coupling agent to form azo dyes with diazotized cephalosporins, allowing for colorimetric detection.

From: "Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde", Arabian Journal of Chemistry, Volume 9, Supplement 2, November 2016, Pages S1272-S1282
Answer
DMAB serves as a coupling agent that reacts with diazotized cephalosporins to form azo dyes. These azo dyes absorb light in the visible region (400–430 nm), enabling spectrophotometric analysis. The use of DMAB allows for a simple, cost-effective, and accurate method for determining cephalosporins in bulk samples and pharmaceutical dosage forms.
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