What is the new spectrophotometric method developed for analyzing cephalosporins, and how does it work?

Label:chem

Topic
Cephalosporins are a class of β-lactam antibiotics widely used for treating various infections. Traditional methods for analyzing cephalosporins, such as HPLC, are time-consuming and expensive. This study aims to develop a simpler, more cost-effective method for the analysis of cephalosporins using spectrophotometry.

From: "Novel spectrophotometric determinations of some cephalosporins following azo dye formation with p-dimethylaminobenzaldehyde", Arabian Journal of Chemistry, Volume 9, Supplement 2, November 2016, Pages S1272-S1282
Answer
The new method involves a two-step process: diazotization of cephalosporins with acidified NaNO₂ at 0–5°C, followed by coupling with acidified p-dimethylaminobenzaldehyde (DMAB) in methanol. The resulting azo adducts absorb light optimally at 400–430 nm, allowing for spectrophotometric analysis. This method is simple, accurate, and cost-effective, making it suitable for quality control in pharmaceuticals.
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