How does calyculin A (cal A) affect apoptosis in human umbilical cord blood lymphocytes (UCBL)?
Label:chem
Topic
Apoptosis is a form of programmed cell death that can be triggered by various stressors, including DNA damage. Calyculin A (cal A) is known to induce apoptosis in certain cell types, but its effects on UCBL have not been thoroughly investigated.
Answer
Cal A (10 nM) induced apoptosis in UCBL within 2 hours of treatment, independent of the delivered radiation dose. Apoptosis was also detected in UCBL treated with 1 nM cal A at longer incubation times (20 and 44 hours). The study suggests that cal A-induced apoptosis may underlie the failure of cal A to maintain radiation-induced γH2AX foci.
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