What is the effect of calyculin A (cal A) on γH2AX DNA repair foci in irradiated human umbilical cord blood lymphocytes (UCBL)?

Label:chem

Topic
Calyculin A (cal A) is a dephosphorylation inhibitor that has been reported to inhibit the disappearance of radiation-induced γH2AX DNA repair foci in human lymphocytes. γH2AX is a molecular marker for DNA double-strand breaks (DSBs), which are critical lesions that can lead to cell death and genetic instability if not properly repaired.
Answer
In a study, cal A (1 and 10 nM) did not stabilize γH2AX foci 2 hours post-irradiation in UCBL. Instead, 10 nM cal A significantly decreased the number of γH2AX foci induced by high-dose (100 cGy) irradiation. This effect was attributed to cal A-induced apoptosis, which may alter chromatin structure and prevent DNA repair proteins from accessing DSB sites.
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