How does calyculin A (cal A) affect the kinetics of DNA repair foci formation in irradiated cells?

Label:chem

Topic
The kinetics of DNA repair foci formation and disappearance are critical for understanding the cellular response to DNA damage. Calyculin A (cal A) has been reported to affect the kinetics of γH2AX foci in some studies, but results have been inconsistent.
Answer
In a study, cal A (1 and 10 nM) did not stabilize γH2AX foci 2 hours post-irradiation. Instead, 10 nM cal A significantly decreased the number of all types of DNA repair foci (γH2AX, 53BP1, and colocalized γH2AX/53BP1) induced by high-dose (100 cGy) irradiation. This suggests that cal A may induce apoptosis-related changes in chromatin structure, preventing DNA repair proteins from accessing DSB sites.
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