How does Olprinone (OLP) affect NF-κB activation in hepatocytes?
Label:chem
Topic
NF-κB is a critical transcription factor involved in the regulation of inflammatory responses and is often activated in liver injury.
Answer
Olprinone (OLP) inhibited the activation of NF-κB in hepatocytes stimulated with IL-1β. This inhibition was observed through electrophoretic mobility shift assays (EMSA), which showed that OLP suppressed the nuclear translocation and DNA binding ability of NF-κB. This effect likely contributes to the reduction of pro-inflammatory cytokines and chemokines, thereby protecting the liver from endotoxin-induced injury.
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