Why is calyculin A considered a significant inhibitor in CRAC pathway research?

Label:chem

Topic
Calyculin A is a type 1/2A serine/threonine phosphatase inhibitor. Initial studies suggested the CRAC pathway was insensitive to it, creating a discrepancy in the understanding of store-regulated Ca2+ uptake (SRCU) regulation.
Answer
Calyculin A, an established inhibitor of type 1/2A serine/threonine phosphatase activity, dramatically inhibits the Ca2+ release-activated Ca2+ (CRAC) entry pathway in intact rat basophilic leukemia (RBL) cells, a finding that re-evaluates its crucial role in controlling CRAC pathway activation and maintenance, contrary to previous reports.
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