Why is 2-APB (2-aminoethoxydiphenyl borate) used in the study of the CRAC pathway?

Label:chem

Topic
2-APB is known to interact with various ion channels and receptors, and specifically with CRAC channels.
Answer
2-APB is utilized as a potent inhibitor of I CRAC in RBL cells; its application results in a marked decline in intracellular Ca2+ concentration to near pre-thapsigargin exposure levels and can reverse thapsigargin-induced membrane depolarization.
Return to Home Chemical List
Knowledge you may be interested in
What is the primary function of thapsigargin in experiments? How does okadaic acid affect the CRAC entry pathway in rat basophilic leukemia cells? Why is calyculin A considered a significant inhibitor in CRAC pathway research? What is Reactive Red 31 (RR31) and why is its degradation in wastewater a significant concern? Why is ceric ammonium nitrate (CAN) considered a versatile reagent for wastewater treatment and organic synthesis? How are cellular protoporphyrin IX levels determined? How is the concentration of coproporphyrin III determined in transport assays? How does succinylacetone (SA) affect hypoxic survival in cells overexpressing ABCB6? Why is dihydroartemisinin (DHA) toxicity affected by free heme levels in leukemia? What is the role of porphyrins in mammalian cells? How does gramicidin-D assist in membrane potential measurements? Why is ferrocene suitable as a catalyst in Fenton reactions? How was ferrocenylmethyl acrylate (FcMA) synthesized? What is the role of ferrocene in the modified membrane? Why was Methyl Orange chosen as a model pollutant for degradation studies? How efficiently was Methyl Orange degraded by the M2 membrane? What is humic acid and its effect on membrane performance? What is the role of Polyacrylonitrile (PAN) in the membrane? What is the role of Methoxy-polyethylene glycol acrylate (PEG 480) in the modified membrane? What is the role of Hydrogen Peroxide in the Fenton reaction?