How is the concentration of coproporphyrin III determined in transport assays?

Label:chem

Topic
Mitochondria are resuspended in buffer, reactions are started, and fluorescence is measured at specific wavelengths.
Answer
The concentration of coproporphyrin III (CP) is determined from a standard curve of CP in 1% NP-40, after lysing pellets by incubation in 1% NP-40 solution and measuring fluorescence at excitation wavelength 405 nm and emission wavelength 630 nm.
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