How are cellular protoporphyrin IX levels determined?

Label:chem

Topic

PPIX is the penultimate precursor of heme biosynthesis. Its levels are determined by fluorescence activated cell sorting.

Answer

Cellular protoporphyrin IX levels are determined by fluorescence activated cell sorting, by washing cells with PBS and analyzing them using a BD LSR II flow cytometer at E×405 nm and Em 695/40 nm for PPIX fluorescence signals.

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