How does elastin-like polypeptides (ELPs) fusion improve the soluble expression of acetaldehyde lyase (ALS)?

Label:chem

Topic
Acetaldehyde lyase (ALS) is an enzyme used in the production of acetoin, a compound with various industrial applications. However, the expression of ALS in Escherichia coli often results in insoluble inclusion bodies, which complicates purification and reduces enzyme yield. Elastin-like polypeptides (ELPs) are thermally responsive biopolymers that can enhance the solubility of fusion proteins.

From: "Improvement of Soluble Expression, Stability, and Activity of Acetaldehyde Lyase by Elastin-like Polypeptides Fusion for Acetoin Production from Acetaldehyde", Biomolecules 2025, 15(9), 1216;
Answer
The fusion of ALS with ELPs significantly improves the soluble expression of ALS in E. coli. The ELP-ALS fusion protein demonstrated 96.8% soluble expression, compared to only 36.9% for His-ALS. This improvement is attributed to the ELPs facilitating proper protein folding and preventing aggregation of the nascent polypeptides, thereby enhancing solubility.
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