What is the effect of meta-Topolin (mT) on the genetic integrity of in vitro propagated sweet potato plants?

Label:chem

Topic
High concentrations of cytokinins can potentially lead to somaclonal variations, which are genetic changes that occur during tissue culture. This study evaluates the genetic integrity of sweet potato plants propagated using different concentrations of meta-Topolin (mT) to ensure that the in vitro propagation protocol maintains genetic uniformity.
Answer
The genetic integrity of in vitro propagated sweet potato plants was maintained across all tested concentrations of meta-Topolin (mT). The study used 48 ISSR primers to assess genetic fidelity and found that all successful primers (32) yielded monomorphic bands in both mother plants and tested plants, confirming the uniformity and genetic integrity of the regenerated sweet potato plants. This indicates that mT concentrations up to 20.73 mM can be safely used for in vitro multiplication without causing genetic variations.
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