How does biotin supplementation interfere with laboratory allergy diagnostics?

Label:chem

Topic
Many immunoassays for total and allergen-specific IgE rely on biotin–streptavidin chemistry, making them susceptible to interference.
Answer
High circulating biotin from supplements can block streptavidin/avidin binding sites, yielding false-negative or false-positive results in assays such as IMMULITE-2000 or NOVEOS; ImmunoCAP systems are unaffected because they do not use biotin–streptavidin bridges. Patients should therefore stop biotin supplementation 48 h (or longer at high doses) before blood collection for such tests.
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