What is the role of entecavir (ETV) in inhibiting the phosphorylation of deoxyguanosine (dG) and deoxyadenosine (dA) in mitochondria?
Label:chem
Topic
Entecavir (ETV) is an antiviral nucleoside analog used to treat chronic hepatitis B. A study investigates whether ETV can competitively inhibit the phosphorylation of deoxyguanosine (dG) and deoxyadenosine (dA) by deoxyguanosine kinase (dGK) in isolated mitochondria and perfused rat hearts.
Answer
ETV competitively inhibits the phosphorylation of dG and dA in rat mitochondria. It has an IC50 of 15.3 ± 2.2 μM for inhibiting dG phosphorylation and an IC50 of 0.028 ± 0.006 μM for inhibiting dA phosphorylation. ETV is a more potent inhibitor of dA phosphorylation than dG phosphorylation, suggesting it could affect the synthesis of dATP and potentially lead to mitochondrial toxicity.
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