How was the successful conjugation of pea protein hydrolysates (PPH) and D-xylose confirmed, and what structural changes were observed?

Label:chem

Topic
Study needed to verify that PPH and D-xylose formed covalent bonds via Maillard glycosylation.
Answer
Successful conjugation was confirmed by FTIR (showing shifts in N-H and C=O stretching vibrations), UV spectroscopy (increased absorption at 280 nm), increased molecular weight distribution (from gel chromatography), SEM (morphological changes from porous sheets to compact smooth sheets), and zeta potential (increased from −11.50 mV to −21.37 mV).
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