How does OpCBR1 affect the biosynthesis of camptothecin in Ophiorrhiza pumila?

Label:chem

Topic
Camptothecin is a potent anti-cancer drug produced by several plant species, including Ophiorrhiza pumila. The biosynthetic pathway of camptothecin involves multiple enzymes and transcription factors. OpCBR1 is an ERF transcription factor identified in O. pumila, but its specific role in camptothecin biosynthesis was previously unknown.
Answer
Overexpression of OpCBR1 in O. pumila hairy roots significantly reduces camptothecin biosynthesis by directly repressing the promoter of the OpIO gene, a key gene in the camptothecin biosynthetic pathway. Conversely, knockout of OpCBR1 increases camptothecin production. This indicates that OpCBR1 acts as a negative regulator of camptothecin biosynthesis.
Return to Home Chemical List
Knowledge you may be interested in
What role does the ERF transcription factor OpCBR1 play in the regulation of camptothecin biosynthesis and root growth in Ophiorrhiza pumila? What improvements were made to the SARS-CoV-2 Mpro high-throughput screening (HTS) assay? What is the role of the SARS-CoV-2 main protease (Mpro) in viral replication, and why is it considered a valid drug target? How does the Ti-salen complex derived from the dimeric salen ligand perform in the asymmetric addition of trimethylsilyl cyanide to various aldehydes? What is the role of the dimeric salen ligand derived from L-tartaric acid in the asymmetric addition of trimethylsilyl cyanide to aldehydes? What is the mechanism of action of pyronaridine tetraphosphate against SARS-CoV-2 and other coronaviruses? How does pyronaridine tetraphosphate compare to other antiviral treatments like molnupiravir and nirmatrelvir in terms of efficacy against SARS-CoV-2? What is the role of pyronaridine tetraphosphate in treating infections caused by highly pathogenic coronaviruses such as SARS-CoV-2, SARS-CoV-1, and MERS-CoV? What are the environmental advantages of using sulfuric acid and ferric sulfate over nitric acid in the leaching process? How does the concentration of sulfuric acid and ferric sulfate affect the leaching efficiency of silver and copper? What is the impact of OpCBR1 on root growth in Ophiorrhiza pumila? How does OpCBR1 regulate the expression of camptothecin biosynthetic genes in Ophiorrhiza pumila? What are the potential applications of OpCBR1 in improving camptothecin production in Ophiorrhiza pumila? What is the potential role of camptothecin in treating breast cancer, particularly HER2-positive breast cancer? How does camptothecin interact with the EGFR receptor in breast cancer? What are the pharmacokinetic properties of camptothecin, and how do they compare to other anticancer compounds? What is the role of gibberellins (GAs) in the germination of Lomatogonium rotatum seeds? How does GA3 treatment affect the embryo development of Lomatogonium rotatum seeds? What are the optimal conditions for GA3 treatment to enhance seed germination in Lomatogonium rotatum? What are the transcriptional changes induced by GA3 treatment in Lomatogonium rotatum seeds during germination?