meta-Topolin
CAS number: 75737-38-1
Meta-topolin (mT) is a relatively new cytokinin isolated from poplar leaves in 1975 and is closely related to 6-benzyladenine (BA).
Related images
Comparison of the perception of THP and THF substituted 6-(3-hydroxybenzylamino)purine (meta-topolin, mT) and mT by selected cytokinin receptors. The effect of 6-(3-hydroxybenzylamino)-9-tetrahydropyran-2-ylpurine (6(3OHBA)9THPP) and 6-(3-hydroxybenzylamino)-9-tetrahydrofuran-2-ylpurine (6(3OHBA)9THFP) on the sensing of mT by the cytokinin receptors CRE1/AHK4 (A) and AHK3 (B). trans-Zeatin (tZ) was used as the positive control.
Related Questions and Answers
A: Meta-topolin was found to be the most effective cytokinin for inducing sufficient multiplication (3.28 shoots/explant) and high-quality shoots with lengths of 1.46 cm when applied at a concentration of 4 µM. It also increased shoot fresh weight and shoot length at 4 and 8 µM, respectively, resulting in high-quality in vitro developed shoots with large, well-expanded leaves. Meta-topolin is recommended as an alternative cytokinin for multiplication of in vitro shoots of high quality from the axillary bud of Húsvéti Rozmaring apple at 4 µM and for shoot elongation at 8 µM.
A: The genetic integrity of in vitro propagated sweet potato plants was maintained across all tested concentrations of meta-Topolin (mT). The study used 48 ISSR primers to assess genetic fidelity and found that all successful primers (32) yielded monomorphic bands in both mother plants and tested plants, confirming the uniformity and genetic integrity of the regenerated sweet potato plants. This indicates that mT concentrations up to 20.73 mM can be safely used for in vitro multiplication without causing genetic variations.
A: The combination of meta-Topolin (mT) with 0.54 mM NAA and 0.09 mM GA3 resulted in the best multiplication response. The study found that the number of shoots significantly increased with increasing mT concentration up to 8.29 mM, with the highest number of shoots (7.26 ± 0.17) and axillary nodes (65.25 ± 1.81) observed at this concentration. This combination provided a robust protocol for in vitro propagation of sweet potato, demonstrating the effectiveness of mT in enhancing shoot multiplication when used with other growth regulators.
Q: What is the role of meta-Topolin (mT) in the in vitro propagation of sweet potato (Ipomoea batatas)?
A: Meta-Topolin (mT) significantly enhances shoot induction and multiplication in sweet potato. The study found that 100% shoot induction was achieved with mT concentrations ranging from 3.11 to 16.58 mM (except 4.15 mM). The best multiplication response, with the maximum number of shoots (7.26 ± 0.17) and axillary nodes (65.25 ± 1.81), was observed on MS medium containing 8.29 mM mT. This indicates that mT can be effectively used to improve the in vitro propagation efficiency of sweet potato.