Protease substrate trials. Representative results for chymotrypsin, pepsin, papain, and thermolysin experiments.

Specific monitoring of chymotrypsin activity in a dual substrate peptide. Incubation of a thioamide/7-methoxycoumarin labeled peptide with both chymotrypsin and trypsin cleavage sites results in a gain of fluorescence only in the presence of chymotrypsin.

Crystal structure of compound 16 bound to the chymotrypsin-like site of the 20S proteasome