Blue and green tppts (2.0 mM) or cysteine (1.0 mM) were added to FeIII(TPPS) (1, 10 μM) and nitrite (10 mM) solutions, respectively, and the reactions were monitored by using NOA monitoring to determine NO formation (dots) and by time absorbance at 413 nm, λmax of FeII(TPPS)(NO) (2) (lines).

Synthesis of TP10-SS-Flu-ITAM (5). The TP10-carrier was first prepared on the resin with the Fmoc-Lys(Boc-Cys(Trt))-OH (20) building block incorporated into the sequence (seventh residue from the N-terminus). During cleavage and deprotection the cysteine was activated, yielding TP10-Cys(pNpys) (22). This carrier was conjugated to cargo Flu-ITAM (30) to yield the construct 5.